三种海水蟹类血淋巴细胞的比较Comparison of Hemocytes with Three Species of Sea Crabs
周晖,谢数涛
摘要(Abstract)：
采用Wright’s-Geimsa混合染料对三疣梭子蟹、锈斑蟳和锯缘青蟹的血淋巴细胞进行染色,光镜下对这三种海水蟹的血淋巴细胞进行了观察和比较。根据血淋巴细胞的核质比、细胞整体着色特点、细胞质中颗粒的存在与否、颗粒的数量和分布等,三种海水蟹的血淋巴细胞均可分为颗粒细胞、半颗粒细胞和透明细胞三种主要类型,三种蟹的同类血淋巴细胞在染色形态上稍有差别。三种蟹的颗粒细胞大小差异较大,而半颗粒细胞和透明细胞大小相近。三种蟹的血淋巴细胞均以半颗粒细胞为主,透明细胞在血淋巴细胞中所占比例最小。三种蟹的血淋巴细胞密度均存在明显的雌雄差异,锈斑蟳和锯缘青蟹血淋巴细胞分类计数也存在明显的雌雄差异。
关键词(KeyWords)： 三疣梭子蟹;锈斑蟳;锯缘青蟹;血淋巴细胞
基金项目(Foundation):
作者(Author): 周晖,谢数涛
参考文献(References)：
- [1]S?derh?ll K,Smith V J,Johansson M W.Exocytosis and uptake of bacteria by isolated haemocyte populations of two crustaceans:evidence for cellular co-operation in the defence reactions of arthropods[J].Cell and tissue research,1986,245:43-49.
- [2]Hose J E,Martin G G,Gerard A S.A decapod hemocyte classification scheme integrating morphology,cytochemistry,and function[J].Biological bulletin,1990,178:33-45.
- [3]Smith V J,Ratcliffe N A.Hose defense reactions of the shore crabs Carcinus maenus in vitro[J].J Mar Biol Assoc U K,1978,58:357-359.
- [4]Bodammer J E.Cytological observation on the blood and hemopoietic tissue in the crab Callinectes sapidus[J].Cell Tiss Res,1978,187:79-96.
- [5]Vaca L L,Fingerman M.The less of hemocytes in tanning during the molting cycle:a histochemical study of the fiddler crab,Uca pugilator[J].Biol Bull,1983,164:50-61.
- [6]Hose J E,Martin G G,Gerard A S.A decapod hemocyte classification scheme integrating morphology,cytochemistry and function[J].Biol Bull,1990,178:33-45.
- [7]Smith V J,Chisholm J R S.Non-cellular immunity in crustaceans[J].Fish&shellfish immunology,1992(2):1-31.
- [8]成永旭,堵南山,赖伟.河蟹血淋巴细胞的超微结构观察[J].华东师范大学学报(自然科学版),1998(3):110-112.
- [9]周玉.日本绒螯蟹血淋巴细胞的研究[J].水生生物学报,2003,27(6):664-666.
- [10]陆宏达.中华绒螯蟹血淋巴细胞的显微、亚显微形态结构及其分类[J].水生生物学报,2002,26(5):494-500.
- [11]朱宁宁,王文,李正荣,等.中华绒螯蟹血淋巴细胞的超微结构[J].中国水产科学,2002,9(2):114-116.
- [12]张凤英,王进科.中华绒螯蟹血淋巴细胞观察[J].水产养殖,2002(5):30-32.
- [13]周玉,穆占昆,杨振国.三疣梭子蟹血淋巴细胞的形态、分类及计数[J].上海水产大学学报,2001,10(3):279-281.
- [14]陈惠群,金珊,王国良,等.三疣梭子蟹血淋巴细胞及血液生化指标的初步研究[J].水产科学,2004,23(6):1-4.
- [15]Johnston M A,Davies P S.Cytology of carcinus hemocytes and their ruction in carbohydrate metabolism[J].Comp Biochem Physiol,1973,46A:569-581.
- [16]Williams A J,Lutz P L.Blood cell types in Carcinus maenus and their physiological role[J].J Mar Biol Assoc U K,1975,55:667-670.
- [17]杨景山.医学细胞化学与细胞生物技术[M].北京:北京医科大学、中国协和医科大学联合出版社,1990:21-26.