颜辰瑶,彭超,吴一超,李顺,刘丹,钱永明,路璐

• 1:西华师范大学生命科学学院
• 2:中国科学院城市环境研究所
• 3:西华师范大学环境科学与工程学院

摘要(Abstract)：

【目的】探究化学分散剂Corexit 9500对海水石油降解微生物及降解效率的影响。【方法】分别设置添加石油、Corexit 9500、石油和Corexit 9500混合物等处理组，采用化学分析及基于16S rRNA基因的高通量测序方法，研究Corexit 9500对海水石油降解过程及细菌群落结构的影响。【结果】培养7 d时，添加Corexit 9500对石油组分总烷烃和总多环芳烃的降解无显著促进作用（P> 0.05）；培养21 d时，添加Corexit 9500显著促进石油组分降解（P <0.05），相比于仅添加石油的处理组，总烷烃和总多环芳烃的降解量分别提高了33.9%和22.0%。测序结果表明，在仅添加石油的处理组中，深圳大鹏湾海水的天然石油降解菌群为海滨海泥杆菌属（Lutimaribacter）、不动杆菌属（Acinetobacter）和浮霉状菌属（Planctomyces），而同时添加Corexit 9500后，石油降解的优势菌群则变为海王球菌属（Neptuniibacter）、Planctomyces和可变单胞菌属（Alteromonas）。仅加入Corexit 9500的处理组也促进了Alteromonas、Lutimaribacter、河口杆菌属（Aestuariibacter）和Ploycyclovorans等石油降解菌富集。【结论】化学分散剂通过改变海水中降解石油的菌群，进而影响细菌对石油的降解过程。

关键词(KeyWords)： 化学分散剂;海洋石油微生物降解;细菌群落结构;降解效率

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（42107278）;; 四川省科技厅应用基础研究项目（2020YJ0346）;; 西华师范大学留学博后基金（416793）

作者(Author): 颜辰瑶,彭超,吴一超,李顺,刘丹,钱永明,路璐

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