盐度对莫荷罗非鱼幼鱼呼吸和氨氮排泄的影响Effects of Salinity on Oxygen Consumption Rate and Ammonia Excretion Rate of Juvenile of Hybrid Tilapia(Oreochromis mossambicus × O. urolepis hornorum)
李明昊,陈刚,黄建盛,张健东,周晖,汤保贵,王忠良,卢迈新,李瑞伟
摘要(Abstract):
将莫荷罗非鱼(Oreochromis mossambicus×O.urolepis hornorum)幼鱼养殖水体盐度从淡水渐变至12、18、24、30(每天提升6)等4个目标盐度,利用间歇式呼吸仪分析幼鱼在目标盐度下5、10、15、20 d时的呼吸和氨氮排泄的变化。结果表明,在实验前期,莫荷罗非鱼幼鱼耗氧率、排氨率随盐度的升高而增加,各盐度处理组均在5 d时升到最大值,之后开始下降,下降时间、幅度与外界盐度有关。盐度12、18组幼鱼耗氧率10 d时下降至对照组水平,20 d时低于对照组水平,差异有统计学意义(P<0.05);盐度24、30组幼鱼耗氧率则在15 d时下降至对照组水平并维持稳定;幼鱼排氨率随实验时间的延长,各盐度处理组幼鱼排氨率呈先上后下降的变化,盐度24、30组幼鱼排氨率在15 d下降并维持在对照组水平,盐度12、18组幼鱼排氨率在15 d时下降至对照组水平并于20 d时低于其他盐度组,差异有统计学意义(P<0.05)。莫荷罗非鱼幼鱼适应新盐度环境后,盐度18组的耗氧率、排氨率最低,与盐度12组差异无统计学意义(P>0.05),与其他盐度处理组有差异有统计学意义(P<0.05),推测其等渗点在盐度12~18之间。不同盐度条件下,氧氮比值介于18.810~24.216间,氨熵介于0.083~0.108间,表明莫荷罗非鱼幼鱼主要依靠蛋白质和脂肪氧化供能。
关键词(KeyWords): 莫荷罗非鱼;盐度;耗氧率;排氨率
基金项目(Foundation): “十二五”国家科技支撑计划项目(2012BAD26B03);; 现代农业产业技术体系专项资金资助(CARS-49);; 广东省科技计划项目“营仔镇水产养殖与加工产品标准化建设”(2010B080203044);; 广东省专业镇中小微企业服务平台建设项目“公馆镇罗非鱼技术创新服务平台建设”(2012B091400016);; 国家农业科技成果转化资金项目“罗非鱼产业关键技术中试与示范”(2010GB2E000335);; 广东省海洋渔业科技推广专项项目“罗非鱼产业发展关键技术研究”(200899B02);; 广东省科技厅重大项目——省部产学研合作专项“罗非鱼产业推进关键技术研究与示范”(2008A020100006)
作者(Author): 李明昊,陈刚,黄建盛,张健东,周晖,汤保贵,王忠良,卢迈新,李瑞伟
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