王海阳,黄郁葱,丁燏,朱帆,简纪常,吴灶和

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点实验室
• 3:广东省高等学校水产经济动物病害控制重点实验室
• 4:仲恺农业工程学院

摘要(Abstract)：

通过同源引物从致病性哈维氏弧菌(Vibrio harveyi)ZJ0603基因组中克隆GST的开放阅读框(ORF),构建真核表达质粒pcDNA-GST。大量抽提重组质粒后,于背鳍基部肌肉注射重组质粒免疫斜带石斑鱼(Epinephelus coioides),分析重组质粒的免疫效果。通过核酸水平检测重组质粒在鱼体肝、肌肉、头肾和脾脏组织的分布;用ELISA法检测鱼体血清的抗体水平,用Western-blot检测目的蛋白的表达情况。结果表明:该序列全长615 bp;免疫7 d后,鱼体中均有质粒分布;斜带石斑鱼血清中产生抗GST的高效抗体(1∶4 096);相应的目的蛋白也在鱼体中成功表达。攻毒后,疫苗免疫保护率达80%,表明GST可作为防治哈维氏弧菌病有效候选抗原。

关键词(KeyWords)： 哈维氏弧菌;谷胱甘肽硫转移酶;斜带石斑鱼;核酸疫苗

Abstract:

Keywords:

基金项目(Foundation): 国家支撑计划(2012BAD17B03);; 广东省海洋经济创新发展区域示范专项(GD2012-B01-004)

作者(Author): 王海阳,黄郁葱,丁燏,朱帆,简纪常,吴灶和

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