陈树河,常云胜,刘晖晖,周维,丁燏

• 1:广东海洋大学水产学院//广东省水产经济动物病原生物学及流行病学重点实验室

摘要(Abstract)：

研究非适宜的p H、温度和盐度条件,以及抗生素胁迫和添加外源H2S条件下溶藻弧菌胱硫醚-β-合成酶(cystathionine-β-synthase,CBS)、超氧化物歧化酶(superoxide dismutase,SOD)和过氧化物酶(peroxidase,POD)的活力及谷胱甘肽(glutathione,GSH)含量的变化,分析CBS与抗氧化系统的相关性,探讨不良环境对CBS活性及抗氧化系统的影响。结果表明,在非适宜温度、盐度及抗生素胁迫下,溶藻弧菌的CBS活力均高于对照组,差异有统计学意义(P<0.05)。在非适宜温度下,其SOD和POD的活力及GSH含量均有不同程度的提高(P<0.05)。在非适宜盐度下,GSH含量上调(P<0.05)。在红霉素和壮观霉素胁迫下,SOD和POD活力均上调(P<0.05),而庆大霉素和四环素胁迫则分别提高SOD、POD活力(P<0.05)。在非适宜p H条件下,低p H上调CBS、SOD和POD的活力及GSH含量(P<0.05);高p H条件下CBS活力与对照组差异无统计学意义(P>0.05),SOD活力较对照组下降(P<0.05)。相关性分析发现,在不同温度、盐度条件下及抗生素胁迫下CBS活力与SOD、POD活力及GSH含量之间具有显著正相关(P<0.05),而在不同p H条件以及抗生素+Na HS处理后,CBS活力与SOD、POD活力及GSH含量之间的相关性无统计学意义(P>0.05)。溶藻弧菌可通过提高CBS活力以调节其抗氧化系统,从而抵御不良环境,而外源H2S则提高溶藻弧菌SOD活力和GSH水平以维持溶藻弧菌正常生理活动。

关键词(KeyWords)： 不良环境;溶藻弧菌;胱硫醚-β-合成酶;抗氧化系统

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金(2014A030313604);; 广东海洋大学“创新强校工程”2013年省奖补资金支持建设项目——高校重大科研项目培育计划(第3类)(Q14196(2013050207));; 广东省教育厅高等学校高层次人才项目“谷胱甘肽及其合成酶系在哈氏弧菌耐药中的作用机制研究”

作者(Author): 陈树河,常云胜,刘晖晖,周维,丁燏

参考文献(References)：

• [1]DING Y,LIU Y,JIAN J C,et al.Molecular cloning and expression analysis of glutathione reductase gene in Chlamydomonas sp.ICE-L from Antarctica[J].Marine Genomics,2012,5:59-64.
• [2]付瑞燕,陈坚,李寅.谷胱甘肽/谷胱甘肽过氧化物酶系统在微生物细胞抗氧胁迫系统中的作用[J].生物工程学报,2007,23(5):771-774.
• [3]CASTELLANO I,MARO A D,RUOCCO M R,et al.Psychrophilic superoxide dismutase from Pseudoalteromonas haloplanktis:biochemical characterization and identification of a highly reactive cysteine residue[J].Biochimie,2006,88(10):1377-1389.
• [4]BARLOY-HUBLER F,CHERON A,HELLEGOUARCH A,et al.Smc01944,a secreted peroxidase induced by oxidative stresses in Sinorhizobium meliloti 1021[J].Microbiology,2004,150(3):657-664.
• [5]CARMEL-HAREL O,STORZ G.Roles of the glutathione-and thioredoxin-dependent reduction systems in the Escherichia coli and Saccharomyces cerevisiae responses to oxidative stress[J].Annual Reviews in Microbiology,2000,54(1):439-461.
• [6]SHATALIN K,SHATALINA E,MIRONOV A,et al.H2S:A universal defense against antibiotics in bacteria[J].Science,2011,334:986-989.
• [7]ARDI?N,OZYURT M.Case report:Otitis due to Vibrio alginolyticus[J].Mikrobiyoloji Bülteni,2004,38(1/2):145-148.
• [8]LIU P C,LIN J Y,HSIAO P T,et al.Isolation and characterization of pathogenic Vibrio alginolyticus from diseased cobia Rachycentron canadum[J].Journal of Basic Microbiology,2004,44(1):23-28.
• [9]AL-OTHRUBI S M Y,CHEAH Y K,MIRHOSSEINI H,et al.Antibiotic resistance of Vibrio parahaemolyticus Isolated from cockles and shrimp sea food marketed in Selangor,Malaysia[J].Clinical Microbiology,2014,3(3):1-7.
• [10]AIASSA V,BARNES A I,ALBESA I.Resistance to ciprofloxacin by enhancement of antioxidant defenses in biofilm and planktonic Proteus mirabilis[J].Biochemical and Biophysical Research Communications,2010,393:84-88.
• [11]郑玉琦.溶藻弧菌耐四种抗生素的蛋白质组学研究[D].湛江:广东海洋大学,2011.
• [12]LIU W H,AU D W T,ANDERSON D M,et al.Effects of nutrients,salinity,p H and light:dark cycle on the production of reactive oxygen species in the alga Chattonella marina[J].Journal of Experimental Marine Biology and Ecology,2007,346(1/2):76-86.
• [13]ZHOU J,WANG W N,MA G Z,et al.Gene expression of ferritin in tissue of the Pacific white shrimp,Litopenaeus vannamei after exposure to p H stress[J].Aquaculture,2008,275(1-4):356-360.
• [14]钱荣华.溶藻弧菌主要毒力相关基因的克隆、表达及其免疫原性研究[D].杭州:浙江大学,2007.
• [15]文春根,张丽红,胡宝庆,等.p H对背角无齿蚌(Anodonta oodiana)5种免疫因子的影响[J].南昌大学学报(理科版),2009,33(2):173-176.
• [16]张桐,徐奇友,许红,等.不同温度下不同蛋白水平对镜鲤(Cyprinus carpio L.)非特异性免疫的影响[J].东北农业大学学报,2010,41(12):80-85.
• [17]CHINNUSAMY V,ZHU J,ZHU J K.Cold stress regulation of gene expression in plants[J].Trends in Plant Science,2007,12(10):444-451.
• [18]KRASENSKY J,JONAK C.Drought,salt,and temperature stress-induced metabolic rearrangements and regulatory networks[J].Journal of Experimental Botany,2012,63(4):1593-1608.
• [19]MCCLUNG C R,DAVIS S J.Ambient thermometers in plants:from physiological outputs towards mechanisms of thermal sensing[J].Current Biology,2010,20(24):R1086-R1092.
• [20]RUELLAND E,ZACHOWSKI A.How plants sense temperature[J].Environmental and Experimental Botany,2010,69(3):225-232.
• [21]STROM A R,KAASEN I.Trehalose metabolism in Escherichia coli:stress protection and stress regulation of gene expression[J].Molecular Microbiology,1993,8(2):205-210.
• [22]WINSTON G W.Oxidants and antioxidants in aquatic animals[J].Comparative Biochemistry and Physiology Part C:Comparative Pharmacology,1991,100(1):173-176.
• [23]H?RMANSDORFER S,WENTGES H,NEUGEBAUR-BüCHLER K,et al.Isolation of Vibrio alginolyticus from seawater aquaria[J].International Journal of Hygiene and Environmental Health,2000,203(2):169-175.
• [24]DU M,CHEN J,ZHANG X,et al.Characerization and resuscitation of viable but nonculturable Vibrio alginolyticus VIB283[J].Archives of Microbiology,2007,188(3):283-288.
• [25]周泽军.溶藻弧菌Ⅲ型分泌系统分子伴侣护航蛋白Vsc O的功能研究[D].湛江:广东海洋大学,2013.
• [26]YE L,JIANG S,ZHU X,et al.Effects of salinity on growth and energy budget of juvenile Penaeus monodon[J].Aquaculture,2009,290(1):140-144.
• [27]PILLAI B R,DIWAN A D.Effects of acutesalinity stress on oxygen consumption and ammonia excretion rates of the marine shrimp Metapenaeus monoceros[J].Journal of Crustacean Biology,2002,22(1):45-52.
• [28]孙鹏,彭士明,尹飞,等.盐度对条石鲷幼鱼Na+/-K+-ATP酶活力的影响[J].水产学报,2010,38(8):1204-1209.
• [29]LI L,ROSE P,MOORE P K.Hydrogen sulfide and cell signaling[J].Annu Rev Pharmacol Toxicol,2011,51:169-187.
• [30]MOLS M,ABEE T.Primary and secondary oxidative stress in Bacillus[J].Environmental Microbiology,2011,13(6):1387-1394.
• [31]QABAZARD B,LI L,GRUBER J,et al.Hydrogen sulfide is an endogenous regulator of aging in Caenorhabditis elegans[J].Antioxidants&Redox Signaling,2014,20(16):2621-2630.
• [32]ELROD J W,CALVERT J W,MORRISON J,et al.Hydrogen sulfide attenuates myocardial ischemiareperfusion injury by preservation of mitochondrial function[J].Proceedings of the National Academy of Sciences,2007,104(39):15560-15565.