赵子涵,王姿曼,邓岳文,杨创业,李俊辉

• 1:广东海洋大学水产学院
• 2:广东省珍珠科技创新中心

摘要(Abstract)：

【目的】克隆大珠母贝(Pinctada maxima)胰岛素相关肽受体PmIRR基因,并分析其在不同组织中的表达。【方法】利用c DNA快速末端克隆技术(RACE)获得大珠母贝PmIRR基因cDNA全长序列。荧光定量PCR技术分析PmIRR在闭壳肌、鳃、外套膜边缘区、套膜区、中央区、足和肝胰腺等组织的表达模式。【结果与结论】PmIRR基因全长6 009 bp,5′非编码区(UTR)615 bp、3′UTR 764 bp、开放阅读框(ORF)长4 629 bp、编码1 542个氨基酸。序列分析表明,PmIRR含有保守结构域Fu、3个FNⅢ结构域和Tyrkc结构域,并含有信号肽和2个跨膜结构域。PmIRR在大珠母贝各个组织存在差异表达,在鳃、肝胰腺和外套膜边缘区中显著高表达。

关键词(KeyWords)： 大珠母贝;胰岛素肽相关受体;基因克隆;表达模式

Abstract:

Keywords:

基金项目(Foundation): 农业产业技术体系专项(CARS-49);; 广东省教育厅珍珠研究创新团队(2017KCXTD016);; 广东省农业农村厅现代农业产业技术创新团队专项(2019KJ146)

作者(Author): 赵子涵,王姿曼,邓岳文,杨创业,李俊辉

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