程波,舒秀君,宋蓓,任源远,韩刚,穆迎春,马兵,宋怿

• 1:中国水产科学研究院
• 2:农业部水产品质量安全与营养品质功能评价重点实验室
• 3:上海海洋大学水产与生命学院

摘要(Abstract)：

【目的】厘清甲壳动物体内氨基脲(SEM)物质来源途径及其为内源性物质提法的科学验证现状,以便修订或完善现有法律法规。【方法】综述了甲壳类水产品中SEM来源途径及验证研究。【结果】甲壳类水产品不同组织普遍存在SEM成分,除养殖过程人为非法使用呋喃西林药物外,甲壳类水产品中SEM可能通过产品加工过程次氯酸盐消毒处理,含有偶氮二甲酰胺的面粉裹粉处理,养殖水体和沉积物环境以及水体中藻类及饲料中引入。有关SEM是水生甲壳动物内源性物质的科学提法目前缺乏直接、有效的科学实验证据。【结论】在判定甲壳动物养殖过程是否非法使用硝基呋喃监管过程中,要求产品中SEM不得检出的判定标准过于严格,亟需深入开展甲壳类水产品中SEM来源途径与内源性SEM验证的科学研究,为甲壳类水产品SEM残留限量标准的修订和采样标准的制定提供科学依据。

关键词(KeyWords)： 虾类;蟹类;甲壳类水产品;内源性物质;氨基脲;硝基呋喃类;来源

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项资金项目(2016C009);; 国家自然科学青年基金项目(31502191);; 现代农业产业技术体系(CARS-46)

作者(Author): 程波,舒秀君,宋蓓,任源远,韩刚,穆迎春,马兵,宋怿

参考文献(References)：

• [1]陈昌福,王玉堂.替代硝基呋喃类和孔雀石绿禁用药物的药物筛查报告[J].中国水产,2012(6):58-61.
• [2]Fortieth report of the Joint FAO/WHO Expert Committee on Food Additives(JECFA).Evaluation of Certain Veterinary Drug Residues in Food[R].WHOTechnical Report Series:WHO,1993:32-40.
• [3]中华人民共和国农业部.第193号公告.食品动物禁用的兽药及其它化合物清单[R].北京,2011.
• [4]VASS M,HRUSKA K,FRANEK M.Nitrofuran antibiotics:a review on the application,prohibition and residual analysis[J].Veterinarni medicina,2008,53(9):469-500.
• [5]VASS M,DIBLIKOVA I,CERNOCH I,et al.ELISA for semicarbazide and its application for screening in food contamination[J].Analytica Chimica Acta,2008,608(1):86-94.
• [6]LEITNER A,Z?LLNER P,LINDER W.Determination of the metabolites of nitrofuran antibiotics in animal tissue by high-performance liquid chromatography-tandem mass spectrometry[J].J Chromatograghy A,2001,939(1/2):49-58.
• [7]POINTS J,BURNS D T,WALKER M J.Forensic issues in the analysis of trace nitrofuran veterinary residues in food of animal origin[J].Food Control,2015,50:92-103.
• [8]Commission of the European Communities.Commission Regulation 1442/95 amending Annexes I,II,III and IV of council regulation EEC no.2377/90 laying down a Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin[R].Official Journal of European Communities,1995,143:28.
• [9]中华人民共和国农业部.第235号公告.动物性食品中兽药最高残留限量[R].北京,2002.
• [10]Commission of the European Communities.Commission Decision 2002/657/EC implementing Council Directive96/23/EC concerning the performance of analytical methods and the interpretation of results[J].Official Journal of European Communities,2002,221:8.
• [11]STADLER R H,VERZEGNASSI L,SEEFELDER W,et al.Why semicarbazide(SEM)is not an appropriate marker for the usage of nitrofurazone on agricultural animals[J].Food Additives&Contaminants:Part A,2015,32(11):1842-1850.
• [12]MCCRACKEN R,HANNA B,ENNIS D,et al.The occurrence of semicarbazide in the meat and shell of Bangladeshi fresh-water shrimp[J].Food Chemistry,2013,136(3/4):1562-1567.
• [13]ZHANG X,CHEN S,XU H,et al.Identification and occurrence of endogenous semicarbazide in prawns and crabs from Zhejiang province,China[J].Food Additives&Contaminants:Part A,2016,33(2):252-258.
• [14]于慧娟,李冰,蔡友琼,等.液相色谱-串联质谱法测定甲壳类水产品中氨基脲的含量[J].分析化学,2012,40(10):1530-1535.
• [15]SAARI L,PELTONEN K.Novel source of semicarbazide:levels of semicarbazide in cooked crayfish samples determined by LC/MS/MS[J].Food Additives&Contaminants,2004,21(9):825-832.
• [16]杨先乐.新编渔药手册[M].北京:中国农业大学出版社,2005:125-127.
• [17]李会生,王玲.二氧化氯在水产养殖业中的应用[J].内陆水产,2001,1(1):39-39.
• [18]张仲秋,郑明.畜禽药物使用手册[M].北京:中国农业大学出版社,2000:74.
• [19]HOENICKE K,GATERMANN R,HARTIG L,et al.Formation of semicarbazide(SEM)in food by hypochlorite treatment:is SEM a specific marker for nitrofurazone abuse?[J].Food Additives&Contaminants,2004,21(6):526-537.
• [20]杨曦,李红光.水产品在消毒水作用下产生呋喃西林代谢物的研究[J].食品工业科技,2011(4):158-159.
• [21]顾培基.偶氮二甲酰胺的生产现状、合成及用途[J].上海化工,1998,23(6):40-42.
• [22]BECALSKI A,LAU B P Y,LEWIS D,et al.Semicarbazide formation in azodicarbonamide-treated flour:?A model study[J].Journal of Agricultural and Food Chemistry,2004,52(18):5730-5734.
• [23]KWON J W.Semicarbazide:Natural occurrence and uncertain evidence of its formation from food processing[J].Food Control,2017,72:268-275.
• [24]STADLER R H,MOTTIER P,GUY P,et al.Semicarbazide is a minor thermal decomposition product of azodicarbonamide used in the gaskets of certain food jars[J].Analyst,2004,129(3):276-281.
• [25]DE LA CALLE M B,ANKLAM E.Semicarbazide:occurrence in food products and state-of-the-art in analytical methods used for its determination[J].Analytical and Bioanalytical Chemistry,2005,382(4):968-977.
• [26]We Are Union:OHS.Substances causing/worsening asthma[EB/OL].[2010-08-14].http://www.ohsrep.org.au/hazards/asthma/substances-causingworsening-asthma.
• [27]World Health Organization.Concise International Chemical Assessment Document 16:AZODICARBONAMIDE[R].Geneva:World Health Organization,1999,6.
• [28]U.S.food and Drug administration.CFR-Code of Federal Regulations Title 21[EB/OL].[2009-04-01].https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm?CFRPart=172&showFR=1&subpartNo de=21:3.0.1.1.3.9
• [29]GB 2760-2011食品安全国家标准食品添加剂使用标准[S].北京:中国质检出版社,2011.
• [30]NOONAN G O,BEGLEY T H,DIACHENKO G W.Semicarbazide formation in flour and bread[J].Journal of Agricultural and Food Chemistry,2008,56(6):2064-2067.
• [31]蒋志红,吴晓萍,王明兴,等.偶氮甲酰胺与氨基脲在面粉及面包虾中的形成关系[J].食品科学,2014,35(19):91-95.
• [32]ISLAM M J,LIZA A A,REZA A H M,et al.Source identification and entry pathways of banned antibiotics nitrofuran and chloramphenicol in shrimp value chain of Bangladesh[J].EurAsian Journal of BioSciences,2014,8(8):71-83.
• [33]徐英江,孙玉增,宋秀凯,等.潮河口邻近海域氨基脲污染现状调查研究[J].海洋与湖沼,2010,41(4):538-542.
• [34]CREWS C.Potential natural sources of semicarbazide in honey Fuentes naturales potenciales de semicarbazida en miel[J].Journal of Apicultural Research,2014,53(1):129-140.
• [35]VAN POUCKE C,DETAVERNIER C L,WILLE M,et al.Investigation into the possible natural occurence of semicarbazide in Macrobrachium rosenbergii prawns[J].Journal of Agricultural and Food Chemistry,2011,59(5):2107-2112.
• [36]张睿,张晓燕,吴斌,等.甲壳类水产品中氨基脲的测定和来源分析[J].环境化学,2012,21(6):915-916.
• [37]彭婕,甘金华,陈建武,等.中华绒螯蟹中氨基脲的分布及产生机理分析[J].淡水渔业,2015(4):108-112.
• [38]王鼎南,周凡,李诗言,等.甲壳类水产品中呋喃西林代谢物氨基脲的本底调查及来源分析[J].中国渔业质量与标准,2016,6(6):6-11.