徐田军,刘楚吾,刘丽,董秋芬,杨叶欣

• 1:广东海洋大学海洋生物研究所
• 2:广东海洋大学海洋生物研究所
• 3:广东海洋大学海洋生物研究所
• 4:广东海洋大学海洋生物研究所

摘要(Abstract)：

关键词(KeyWords)： 基因间隔序列(ITS);种质鉴定;遗传多样性

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 徐田军,刘楚吾,刘丽,董秋芬,杨叶欣

参考文献(References)：

• [1]邱芳,伏建民.遗传多样性的分子检测[J].生物多样性,1998,6:143-150.
• [2]陈琳林,孔晓瑜,周立石,等.魁蚶核糖体DNA基因转录间隔区的序列特征[J].中国水产科学,2005,12(1):104-107.
• [3]孙博.海湾扇贝分子标记的研究与应用[D].北京:中国科学院研究生院,2003.
• [4]邓筑虹.四种笛鲷rDNA-ITS1测序和Cytb-PCR-SSCP初步分析[D].湛江:湛江海洋大学,2004.
• [5]丁小雷.双壳纲贝类rDNA分子系统学及马氏珠母贝遗传多样性的研究[D].武汉:武汉大学,2003.
• [6]魏晓华.栉孔扇贝和海湾扇贝的遗传多样性研究及扇贝科几种贝类的分子系统学研究[D].青岛:中国海洋大学,2004.6
• [7]孔晓瑜.栉孔扇贝Chlamys farreri和牡蛎Crassostrea的遗传多样性研究[D].青岛中国海洋大学,2004.
• [8]郑雪松,杨虹,李道棠,等.基因间隔序列(ITS)在细菌分类鉴定和种群分析中的应用[J].应用与环境生态学报,2003,9(6):678-684.
• [9]Garcia-Martinez J,Acinas S G,Anton AI,Rodriguez-Valera F.Useof the 16S-23S ribosomal genes spacer region in studies of prokaryoticdiversity[J].Microbial Meth,1999,36:55-64.
• [10]Aakra A,Utaker JB,Nes I F.RFLP of rRNA genes and sequencingof the 16S-23S rDNA intergenic spacer region of ammonia-oxidiz-ing bacteria:a phylogenetic approach[J].Int J Syst Bacterial,1999,49:123-130.
• [11]Dover G A.Molecular drive:a cohesive mode of species evolution[J].Nature,1982,299:111-117.
• [12]Hillis M D M,Davis S K.Ribosomal DNA:Intraspecific polypor-phism,concered evolution and phylogeny reconstruction[J].SystZool,1988,37:63-66.
• [13]商慧敏.盾纤亚纲(纤毛门,寡膜纲)纤毛虫的分子系统学研究[D].青岛:中国海洋大学,2004.
• [14]Vidigal T H,Kissingler J C,Caldeira R L et al.Phylogenetic rela-tionships among Brazilian Biomphalaria species(Molluscs:Planorbri-dae)based upon analysis of ribosomal ITS2 sequences[J].Parasitolo-gy,2000,121:611-620.
• [15]Verneau O,Renaud F,Catzeflis F.Evolutionary relationships of sib-ling tapeworm species(Cestoda)parasitizing teleost fishes[J].MolBiol Evol,1997,14:630-636.
• [16]Odorica D M,Miller D J.Variation in the ribosomal internal tran-scribed spacers and 5.8S rDNA among five species of Acropora(Cni-daria;Scleractinia):patterns of variation consistent with reticulateevolution[J].Mol Biol Evol,1997,14(5):465-473.
• [17]丁雪娟,李安兴,张剑英,等.海水鱼类寄生本尼登虫的ITSI序列[J].华南师范大学学报,2003(2):85-80.
• [18]Zahler M,Schein E,Rinder H et al.Characteristic genotypes dis-criminate between Babesia isolates of differing vector specificity andpathogenicity to dogs[J].Parasitol Res,1998,84:544-548.
• [19]林瑞庆,董世娟,青楚雄,等.片形吸虫第一内转录间隔区DNA多态性的研究[J].Chinese Journal of Veterinary Science and Tech-nology,2004,34(7):8-13.
• [20]Ana Insua,Maria J Lopez-Pinon,Ruth Freire,et al.Sequenceanalysis of the ribosomal DNA internal transcribed spacer region insome scallop species(Mollusca:Bivalvia:Pectinidae)[J].Genome,2003,46:595-604
• [21]Coleman A W,Vacquier V D.Exploring the phylogenectic utility ofITS sequences for animals:a test ease for abalone(Haliotis)[J].Mol.Evol,2002,54:246-257.
• [22]Ernandez A F,Atcia T G,Sensio L A,et al.PCR-RFLP Analysis ofthe internal transcribed spacer(ITS)region for identification of 3clam species[J].Journal of Food Science,2001,66(5):657-661.
• [23]Phillps R B,Sajdak S L,Michael J.Domanico.Relationships amongcharrs bases on DNA Sequences[J].Nordic J.Frdshw.Res.,1995,71:378-391.
• [24]Ferndndez A,Garcia T Asensio,et al.PCR-RFLP analysis of theinternal transcribed spacer(ITS)region for identification of 3 clamspecies[J].Food.Sci.,2000,66:657-661
• [25]Domanico M J,Phillips R B,Oakley T H.Phylogenetic analysis ofPacific salmon(genus Oncorhynchus)using nuclear and mitochondrialDNA sequences[J].Can.J.Fish.Aquat.Sci.,1997,54:1865-1872.
• [26]Sajdak L S,Ruth B Phillips.Phylogenetic relationships among Core-gonus species inferred from the DNA swquence of the first internaltranscribed spacer(ITS1)of ribosomal DNA[J].Can.J.Fish.Aquat.Sci.,1997,54:1494-1503.
• [27]Presa P,Pardo B G,et al.Phylogeographic Congruence BetweenmtDNA and rDNA ITS Markers in Brown Trout[J].Mol.Biol.Evol.2002,19(12):2161-2175.
• [28]Harris D J,Crandall K A.Intragenomic variation within ITS 1 andITS2 of freshwater crayfishes(Decapioda:Cambaridae):implicationsfor phylogenetic and microsatellite studies[J].Mol Biol Evol,2000,17(2):284-291.
• [29]Chu K H,Li C P,Ho H Y.The first internal transcribed spacer(ITS-1)of Ribosomal DNA as a molecular marker for phyloge netic andpopulation analyses in Crustacea[J].Marine Biotechnology,2001,3(4):355-361
• [30]Heath D D,Rawson P D,Hilbish T J.PCR-based nuclear markersidentify alien bluemussel(Mytilusspp.)genotypes on the west coastof Canada[J].Can.J.Fish.Aquat.Sci.,1995,52:2621-2627.
• [31]姜艳艳.蓝点马鲛和日本的遗传多样性研究[D].青岛:中国海洋大学,2003.
• [32]喻子牛,姜艳艳,孔小瑜,等.栉孔扇贝核糖体DNA转录间隔子序列研究及其前在应用[J].中国水产科学,2001,8(1):6-9.
• [33]King T L,Eachles M S,Gjetvaj B,Hoeh W R.Intraspecific phylo-geography ofLasuigona subviridis(Bivalvia:Unionidae):conserva-tion implications of range discontinuity[J].Mol.Ecol.,1999,8:S65-S78.
• [34]Toro J E.Molecular identification of four species of mussels fromsouthern Chile by PCR-based nuclear markers:the potential use instudies involving planktonic surveys[J].Shellfish Res.,1998.17:1203-1205.
• [35]王献溥.生物多样性保护与利用的主要研究方向[J].自然资源,1994(4):1-6.
• [36]唐伯平,周开亚,宋大祥.核rDNA-ITS区在无脊椎动物分子系统学研究中的应用[J].动物学杂志,2002,37(4):67-73
• [37]Tang B P,Zhou K Y,Xiang D X,et al.Molecular systematics of theAsian mitten crabs,genus Eriocheir(Crustacea:Brachyura)[J].Mo-lecular Phylogenetics and Evolution,2003,29:309-316.
• [38]Tang B P,Zhou K Y,Xiang D X,et al.Study on Size Variation ofrDNA Internal Transcribed Spacer 1 in Eriocheir japonica sinensis(Decapoda,Grapsidae)[J].Journal of East China Normal University(Natural Science),2003,3:61-68.