柯剑;赵飞;罗理;姜兰;卢迈新;邹为民;

• 1:中国水产科学研究院珠江水产研究所
• 2:上海海洋大学水产与生命学院

摘要(Abstract)：

从广东省5个罗非鱼主养区患暴发性的罗非鱼中分离到15株菌,挑选其中的5株(不同地域、不同组织分离的菌株)进行人工感染试验,表现出自然发病的症状,确定此5株分离菌为导致罗非鱼暴发病的主要病原。通过形态学观察和生理生化实验,并结合16SrRNA基因序列分析,5株分离菌株均为无乳链球菌(Streptococcus agalactiae)。5株分离菌在形态学和生理生化实验结果上保持一致,16SrRNA基因序列同源性达98.6%～99.8%,但在致病性和对药物的敏感性上却呈现一定的差异。另外10株分离菌株通过生理生化实验鉴定也为无乳链球菌。研究表明,广东省罗非鱼流行性暴发病的病原菌主要为无乳链球菌。

关键词(KeyWords)： 罗非鱼;无乳链球菌;致病性;细菌鉴定

Abstract:

Keywords:

基金项目(Foundation): 广东省海洋渔业局病害防治专项(2007);; 国家科技支撑计划(2006BAD03B04-02);; 广东省农业重点项目(2009B020201003);; “现代农业产业技术体系建设专项资金”(nycytx-48-4)

作者(Authors): 柯剑;赵飞;罗理;姜兰;卢迈新;邹为民;

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