杨创业,章佳斌,曾业涛,廖永山,邓岳文,王庆恒

• 1:广东海洋大学水产学院
• 2:广东海洋大学珍珠研究所
• 3:广东省珍珠养殖与加工工程技术研究中心

摘要(Abstract)：

【目的】研究血清素(5-HT)、蜕皮激素、γ-氨基丁酸(GABA)、腺嘌呤核苷、次黄嘌呤核苷和次黄嘌呤对马氏珠母贝眼点幼虫附着的影响。【方法】以不同浓度的5-HT、蜕皮激素、GABA、腺嘌呤核苷、次黄嘌呤核苷和次黄嘌呤溶液处理马氏珠母贝眼点幼虫,不同时间后,分析眼点幼虫附着率的变化。【结果】在24～96 h作用时间里,5-HT、蜕皮激素、GABA对眼点幼虫附着的诱导效果整体上均呈上升趋势;在24～120 h作用时间里,腺嘌呤核苷、次黄嘌呤核苷和次黄嘌呤对眼点幼虫附着的诱导效果整体上呈上升趋势。眼点幼虫在10~(-5) mol/L的5-HT和GABA处理24、48、72和96 h后,附着率均显著高于对照组(P <0.01);10~(-5) mol/L的蜕皮激素处理24 h和72 h,10~(-6) mol/L的蜕皮激素处理24 h和96 h,10~(-7) mol/L的蜕皮激素处理24 h,附着率均显著高于对照组(P <0.01);10μmol/L腺嘌呤核苷处理24、48、72和120 h,1μmol/L腺嘌呤核苷处理24、48、72、96和120 h,附着率均显著高于对照组(P <0.01);10μmol/L次黄嘌呤核苷处理48和72 h,1μmol/L次黄嘌呤核苷处理48、72、96和120 h,附着率均显著高于对照组(P <0.01);10μmol/L的次黄嘌呤处理72、96和120 h,附着率均显著高于对照组(P<0.01)。【结论】5-HT、蜕皮激素、GABA、腺嘌呤核苷、次黄嘌呤核苷和次黄嘌呤溶液在适宜的浓度和处理时间均可诱导马氏珠母贝眼点幼虫附着,其中10~(-5) mol/L的5-HT处理24 h对附着率的诱导效果最佳,可达对照组的6.3倍。

关键词(KeyWords)： 马氏珠母贝;眼点幼虫;附着;化学物质

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（32102817）;; 广东省基础与应用基础研究基金项目（2019A1515111026）;; 广东省农业农村厅项目（2020KJ146和粤财农[2020]4号）;; 广东省教育厅项目（2019KQNCX043和2020ZDZX1045）;; 大学生创新创业训练计划项目（CXXL2020045）

作者(Author): 杨创业,章佳斌,曾业涛,廖永山,邓岳文,王庆恒

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