何远法,迟淑艳,谭北平,张含乐,董晓慧,杨奇慧,刘泓宇,章双

• 1:广东海洋大学水产学院
• 2:南海生物资源开发与利用协同创新中心
• 3:北京英惠尔生物技术有限公司

摘要(Abstract)：

在基础饲料中分别添加质量分数0(对照)和0.30%的酵母培养物,配制2种等氮等脂饲料(分别记为Y0、Y0.3组),投喂初始体质量为(1.20±0.01)g的凡纳滨对虾(Litopenaeus vannamei)56 d。提取凡纳滨对虾肠道总DNA,采用Illumina Mi Seq高通量测序比较样品菌群组成和多样性,研究饲料中添加酵母培养物对凡纳滨对虾肠道菌群结构的影响。结果表明,Y0.3组对虾肠道分类单元(OTUs)数量、丰富度指数(Chao1、ACE值)、多样性指数(Shannon、np Shannon指数)与Y0组差异无统计学意义(P>0.05);从物种分类树发现,两组的主要细菌类群均为变形菌门(Proteobacteria),其中,γ-变形菌纲是对虾肠道的最主要菌群,所占比例达69.48%;从科分类水平看,Y0.3组对虾肠道菌群链球菌科(Streptococcaceae)比Y0组提高4.98%;从优势属水平来看,Y0.3组对虾肠道的乳球菌属(Lactococcus)高于Y0组,差异有统计学意义(P<0.01);Y0.3组对虾肠道的发光杆菌属(Photobacterium)低于Y0组,差异有统计学意义(P<0.05)。饲料中添加质量分数0.30%酵母培养物可改善凡纳滨对虾肠道菌群多样性。

关键词(KeyWords)： 酵母培养物;凡纳滨对虾;肠道菌群;菌群结构;多样性;高通量测序

Abstract:

Keywords:

基金项目(Foundation): 农业部公益性行业科研专项(201003020);; 广东省教育厅高等学校高层次人才项目(粤财教[2013]246)

作者(Author): 何远法,迟淑艳,谭北平,张含乐,董晓慧,杨奇慧,刘泓宇,章双

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