运输密度和时间对大口黑鲈幼鱼生理生化和肝肠结构的影响Effects of Transportation Density and Duration on the Physiological and Biochemical Indicators, Liver and Intestine Structure of Juvenile Largemouth Bass(Micropterus salmoides)
王锦绣,王炬光,李伟,白婵,祁雪,李豪华,邱亮,熊光权,廖涛
摘要(Abstract):
【目的】研究不同运输密度和时间对大口黑鲈(Micropterus salmoides)幼鱼生理生化指标及肝肠组织结构的影响,为其合理科学运输提供理论依据。【方法】将大口黑鲈幼鱼置于塑料盒中进行模拟运输实验,分别于运输前(0 h)和运输后的4、8、12、20 h取样,对存活率、水质、血清和肝脏理化指标进行了分析。【结果】与运输前相比,运输过程中水体总氨氮浓度和pH显著升高,即时存活率显著降低。运输密度和时间对大口黑鲈幼鱼即时存活率、水体总氨氮含量、应激及抗氧化指标均具有显著交互作用。1∶2、1∶4、1∶5密度组皮质醇含量在4 h达到最高,1∶3密度组在8 h达到最高,之后逐渐降低。肝脏总抗氧化能力和超氧化物歧化酶与运输前相比显著升高,还原型谷胱甘肽含量显著降低,各密度组肝脏和肠道均出现不同程度的组织损伤(α=0.05)。【结论】运输时长小于4 h时,可适当采用较高的鱼水比(1∶2)运输,运输时长大于10 h时,宜采用较低的鱼水比(1∶5)进行运输。
关键词(KeyWords): 大口黑鲈幼鱼;运输密度;应激指标;即时存活率;组织结构
基金项目(Foundation): 国家现代农业产业技术体系专项资助(CARS-46);; 湖北省农业科技创新中心重大科技研发专项(2020-620-000-002-03)
作者(Author): 王锦绣,王炬光,李伟,白婵,祁雪,李豪华,邱亮,熊光权,廖涛
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