注射氟苯尼考对红笛鲷免疫指标的影响Effects of Florfenicol on Immune Indexes of Crimson Snapper (Lutjanus sanguineus)
张丽敏,吴灶和,简纪常,鲁义善,简贺君
摘要(Abstract)：
以0、5、10、20 mg.kg-1的氟苯尼考腹腔注射红笛鲷,研究氟苯尼考在国家规定的使用范围内对红笛鲷血清部分免疫指标的影响。结果显示:5 mg.kg-1组氟苯尼考对所测的各项指标无影响或影响不显著;10 mg.kg-1组中氟苯尼考对红笛鲷血清SOD活力、AKP活力有显著性提高(P<0.05),对溶菌酶的含量、IgM的含量无显著性影响;20 mg.kg-1组中红笛鲷血清碱性磷酸酶AKP、超氧化物歧化酶SOD活力、溶菌酶、抗体IgM均受到显著性抑制(P<0.05),但影响持续时间不同,这表明高浓度氟苯尼考显著抑制红笛鲷各项免疫指标,从而影响到鱼体自身的免疫功能。
关键词(KeyWords)： 红笛鲷;氟苯尼考;抗体IgM;超氧化物歧化物SOD;碱性磷酸酶AKP;溶菌酶
基金项目(Foundation):
作者(Author): 张丽敏,吴灶和,简纪常,鲁义善,简贺君
参考文献(References)：
- [1]Labro M T.Cefodizime as a biological response modifier:a re-view of its in-vivo,ex-vivo and in-vitro immunomodulatoryproperties[J].Journal of antimicrobial chemotherapy,1990,26:37.
- [2]Labro MT.Immunomodulation by antibacterial agents,is itclinical relevant?[J].Drugs,1993,45:319-328.
- [3]Labro MT.Antibiotics as biological modifiers:from the labora-tory to clinical practice[C].3rdWestern pacific congress onchemotherapy and infectious diseases.Bali,Decmber,1992:6-9.
- [4]Puangkaew J,Kiron V.Nonspecific immune response of rain-bow trout(Oncorhynchus mykissWalbaum)in relation to dif-ferent status of vitamin E and highly unsaturated fatty acids[J].Fish&shellfish immunology,2004,16:25-39.
- [5]Niesen H.Antibiotics and human monocyte functionⅡ[J].Phagocytosis and oxidative metabolism,1989,97:447-451.
- [6]Limbert M,Bartlett RR,Dicknette G,et al.Cefodizime,an ami-nothiazolyl cephalosporin,Influence on the immune system[J].Antibiotic,1984,37(12):1719.
- [7]Anderson R,Grabow G,O sthuizen R,et al.Effect of sulfamethoxazole and trimethoprium on human neutrophil and lym-phocyte functions in-vitro and in-vivo effects of cotrimoxaxzole[J].Antimicrob agents chemother,1980,17:322-326.
- [8]Cuffini AM,Tullio V,Allocco A,et al.Effect of rufloxacinupon non-specific immune defence:in-vitro,ex-vivo and in-vi-vo results[J].Journal of antimicrobial chemotherapy,1994,34(4):545.
- [9]Lunden T,Miettinen S.Effect of florfenicol on the immuneresponse of rainbow trout(Oncorhynchus mykiss)[J].Veteri-nary immunology and immunopathology,1999,67:317-325.
- [10]李永芹,简纪常,吴灶和,等.红鳍笛鲷弧菌病病原的研究[J].湛江海洋大学学报,2004,24(6):2-5.
- [11]中华人民共和国农业部.新编渔药手册[M].北京:中国农业出版社,2005:187-189
- [12]朱立平,陈学清.免疫学常用实验方法[M].北京:人民军医出版社,2000:352-356
- [13]张辉,张海莲.碱性磷酸酶在水产动物中的作用[J].河北渔业,2003,131(5):30-32.
- [14]张凤君.多氯联苯暴露对实验鱼主要器官(组织)微细结构变化及几种酶活性的影响[D].广州:华南师范大学,2002-06:23
- [15]杨玉奇,李云锋,丁生巧,等.苯及其同系物对人体中性粒细胞中碱性磷酸酶活性影响的研究[J].中华医学从刊,2002,2(5):6-7.
- [16]刘斌,周培疆,吴新国,等.联苯胺对碱性磷酸酶抑制作用及其动力学研究[J].环境科技与技术,2005,28(3):6-8.
- [17]余东游,邹晓庭.杆菌肽锌和金霉素对蛋鸡产蛋性能和蛋品质的影响机理[J].浙江大学学报(农业与生命科学版),2000,l.26(2):169-172.
- [18]Roszkowski W,Ko H L,Roszkowski K,et al.Effects ofciprofloxacinon the humoral and cellular immune responses inBalb/c-mice[J].Zentralbl Bacteriol Mikrobiol Hyg,1986,262(3):396.