萧振邦,黄晓洋,陈佳丽,杨胜涛,刘怡,千忠吉

• 1:广东海洋大学化学与环境学院
• 2:广东海洋大学食品科技学院
• 3:广东海洋大学深圳研究院

摘要(Abstract)：

【目的】探究序列为KVEPQDPSEW的鲍鱼肠多肽AATP对LPS诱导HUVEC人脐静脉内皮细胞的抗炎作用。【方法】用"MTT"法检测细胞活力,"DCFH-DA"法测定活性氧(ROS)的含量,用蛋白免疫印迹法检测细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)和内皮素-1(ET-1)以及NF-κB信号通路相关蛋白的表达情况,酶联免疫实验检测白细胞介素-6(IL-6)的释放量,分子对接分析AATP与Toll样受体(TLR4)蛋白的相互作用。【结果】"MMT"法证明AATP对HUVEC细胞无明显毒性作用(P> 0.05),且能提高脂多糖(LPS)诱导组的HUVEC细胞活力(P <0.05);与对照组相比,随着实验组AATP浓度增加,ROS含量明显逐渐减少(P <0.05),内皮炎症因子ICAM-1、VCAM-1、ET-1和IL-6的表达也明显逐渐减少(P <0.05),NF-κB信号通路中p65、I-κBα的磷酸化表达显著下降(P <0.05);对接结果表明,AATP与TLR4能形成稳定的氢键,有相互作用的可能性。【结论】AATP表现出很好的清除ROS作用,可明显抑制HUVEC的炎症因子的表达和NF-κB信号通路的激活。

关键词(KeyWords)： 鲍鱼;多肽;HUVEC;抗炎;脂多糖

Abstract:

Keywords:

基金项目(Foundation): 广东省基础与应用基础研究基金项目（2020A1515011075）

作者(Author): 萧振邦,黄晓洋,陈佳丽,杨胜涛,刘怡,千忠吉

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