金钱鱼Dmrt4基因的克隆及表达分析Molecular Cloning and Expression Profile of Dmrt4 in Spotted Scat(Scatophagus argus)
江东能,彭友幸,奥马尔·法鲁克·穆斯塔法,古皓天,邓思平,陈华谱,朱春华,吴天利,李广丽
摘要(Abstract)：
【目的】研究金钱鱼(Scatophagus argus)Dmrt4(Doublesex and Mab-3 related transcription factors 4)基因在性腺和胚胎发育中的表达。【方法】克隆金钱鱼Dmrt4,采用逆转录PCR检测Dmrt4的组织表达,用实时荧光定量PCR检测Dmrt4在性腺和胚胎发育过程中的表达。【结果与结论】金钱鱼Dmrt4的开放阅读框(ORF)全长1 260 bp,编码蛋白全序列为419个氨基酸。金钱鱼Dmrt4氨基酸序列含有DM和DMA结构域。金钱鱼Dmrt4与欧洲海鲈(Dicentrarchus labrax)和大黄鱼(Larimichthys crocea)的同源性最高,为87.6%;与非洲爪蟾(Xenopus laevis)的同源性最低,为47.1%。金钱鱼Dmrt4与其他鲈形目鱼类在系统进化树上聚为一支。金钱鱼Dmrt4在精巢、卵巢和鳃中高表达,在Ⅲ和Ⅳ期精巢中的表达显著高于V期精巢和Ⅱ、Ⅲ和Ⅳ期卵巢。胚胎发育过程中Dmrt4均有表达,原肠胚的表达水平最高。
关键词(KeyWords)： 金钱鱼;Dmrt4;基因克隆;组织表达;性腺;胚胎发育
基金项目(Foundation): 湛江市财政资金科技竞争性分配项目(2016A03017);; 广东省自然科学基金(2016A030313743);; 国家青年基金(31702326)
作者(Author): 江东能,彭友幸,奥马尔·法鲁克·穆斯塔法,古皓天,邓思平,陈华谱,朱春华,吴天利,李广丽
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