钟绮媚,张晶晶,杨志友,邓淑怡,王佳佳,张才,张永平,宋采

• 1:广东海洋大学食品科技学院
• 2:广东省水产品加工与安全重点实验室
• 3:广东医科大学附属医院
• 4:广东海洋大学深圳研究院

摘要(Abstract)：

【目的】研究ω-3多不饱和脂肪酸（PUFAs）二十碳五烯酸（EPA）、二十二碳五烯酸（DPA）和二十二碳六烯酸（DHA）对铝致斑马鱼认知、记忆损伤和病理变化的影响。【方法】利用共聚焦显微镜观察斑马鱼幼鱼脑内荧光强度。以100μg/L铝诱导斑马鱼阿尔茨海默病（AD）模型，给予EPA、DPA和DHA后，进行新物体识别和T迷宫奖赏测试，Elisa检测β-Amyloid(Aβ)、p-tau、乙酰胆碱酯酶（AChE）和白细胞介素（IL）-1β的含量，qPCR检测小胶质细胞标志物Hexb与星形胶质细胞标志物GFAP及脑源性神经营养因子（BDNF）的变化。【结果】与对照组相比，铝显著激活斑马鱼幼鱼脑内的小胶质细胞。在行为实验中，与对照组相比，铝显著延长到达新臂、奖励臂潜伏期，减少在新臂、奖励臂时间；EPA、DPA和DHA均显著增加在新臂、奖励臂时间；EPA和DHA显著减少到达奖励臂的潜伏期；与对照组相比，模型组显著增加Aβ、p-tau、AChE和IL-1β的蛋白浓度以及Hexb和GFAP的mRNA表达。EPA、DPA和DHA均显著降低Aβ蛋白浓度和GFAP的mRNA表达。EPA显著降低p-tau、AChE和IL-1β的蛋白浓度，并抑制Hexb及上调BDNF的mRNA表达；DPA亦显著降低p-tau蛋白浓度；DHA显著降低AChE浓度。【结论】三种ω-3PUFAs对斑马鱼AD样变化作用各不相同，EPA的效果最佳。

关键词(KeyWords)： 3多不饱和脂肪酸;阿尔茨海默病;神经炎症;斑马鱼;学习记忆

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金（2021A1515011579）;; 深圳市科技计划（国际合作研究）项目(GJHZ20190823111414825);; 广东省科技计划项目(2016B020235001)

作者(Author): 钟绮媚,张晶晶,杨志友,邓淑怡,王佳佳,张才,张永平,宋采

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