刘凡,王雨涵,董苗萍,靳翠丽,刘青,周晓见

• 1:扬州大学环境科学与工程学院

摘要(Abstract)：

【目的】研究光周期和维生素对巴夫藻（Pavlova sp.）生长和物质产量的影响，为提高巴夫藻的培养效益提供实验依据。【方法】采用维生素浓度和光周期的双因素实验，培养巴夫藻14 d，测定藻细胞中色素、脂肪、蛋白质和多糖的含量，并分析脂肪酸组成和生物柴油性能的变化。【结果】最适合巴夫藻生长的光周期是16 h∶8 h。在1V 16 h∶8 h的条件下，巴夫藻的生物量干质量和蛋白质产量最高，分别为501.50和55.54 mg·L~(-1)。在8V 8 h∶16 h条件下，岩藻黄素含量和产量最高，分别为6.21 mg·g~(-1)和2.60 mg·L~(-1)。在8V 24 h∶0 h和8V 16 h∶8 h条件下，脂肪含量和产量最高，分别为30.18%和123.87 mg·L~(-1)。最适合二十碳五烯酸（Eicosapentaenoic acid,EPA）生产的光周期为8 h∶16 h，在1V 8 h∶16 h的条件下，EPA占比达15.46%。在4V 24 h∶0 h条件下，生物柴油的十六烷值（CN）达到53.98，不饱和度（DU）和碘值（IV）最低。【结论】与维生素浓度相比，光周期对巴夫藻培养更为重要，光周期不仅影响巴夫藻的生长，还对其物质生产影响显著，并能改变巴夫藻的脂肪酸组成和生物柴油性能。

关键词(KeyWords)： 巴夫藻(Pavlova sp.);光周期;维生素;生长;物质生产

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（42177459）;; 扬州大学大学生科创基金（XCX20230622）

作者(Author): 刘凡,王雨涵,董苗萍,靳翠丽,刘青,周晓见

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