张克伟,陈华谱,江东能,邓思平,朱春华,吴天利,李广丽

• 1:广东海洋大学水产学院//南海水产经济动物增养殖广东省普通高校重点实验室//广东省名特优鱼类生殖调控与繁育工程技术研究中心//湛江市海洋生态与养殖环境重点实验室

摘要(Abstract)：

【目的】克隆金钱鱼IGF-1和IGF-2基因,研究二基因在金钱鱼胚胎发育过程中的表达。【方法】采用实时荧光定量PCR,以不同发育时间点的金钱鱼(Scatophagus argus)胚胎为实验材料,分析IGF-1和IGF-2基因在鱼类胚胎发育过程中的表达图式。【结果】金钱鱼IGF-1和IGF-2的蛋白全序列分别为186和215个氨基酸。序列分析表明,IGF-1和IGF-2均有胰岛素样生长因子蛋白典型结构,含有N端信号肽、B、C、A、D和E结构域,且有6个保守的半胱氨酸,与其他鲈形目鱼类花鲈的IGF-1和IGF-2相似性较高(95%和91%)。IGF-1从受精卵到出膜的胚胎发育过程中表达量逐渐上调;IGF-2在受精后至囊胚期表达量极低,而在原肠胚期和神经胚期表达量显著上调至最高水平,在神经胚期后表达量显著降低并维持稳定。【结论】IGF-1和IGF-2序列保守性低,与鲈形目同源性高,在金钱鱼胚胎发育过程中起不同调控作用。

关键词(KeyWords)： 金钱鱼;IGF-1;IGF-2;基因克隆;胚胎发育

Abstract:

Keywords:

基金项目(Foundation): 湛江市财政资金科技竞争性分配项目(2016A03017,2017A03019);; 广东省自然科学基金(2016A030313743);; 广东高校省级重点平台和重大科研项目(2015KTSCX058)

作者(Author): 张克伟,陈华谱,江东能,邓思平,朱春华,吴天利,李广丽

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