彭敏,罗邦,朱威霖,杨春玲,赵永贞,李强勇,刘青云,曾地刚,李旻,陈秀荔

• 1:广西壮族自治区水产科学研究院
• 2:广西水产遗传育种与健康养殖重点实验室

摘要(Abstract)：

【目的】了解巨砺属牡蛎的系统关系和种类区分。【方法】对我国沿海分布的7种巨砺属牡蛎(香港牡蛎、有明牡蛎、熊本牡蛎、岩牡蛎、艾氏牡蛎、太平洋牡蛎、葡萄牙牡蛎)的线粒体DNA片段(COI、12S、16S和t RNAs),以及核糖体RNA基因转录间隔子序列(ITS-1、ITS-2和ITS)进行分子系统学研究。【结果】无论种间或种内,t RNAs序列差异最大,亚种内TS1序列差异最大;t RNAs和COI序列较其它序列变异更快,种间变异与种内变异之间有明显的条形码间隙;12S、16S、ITS和ITS2的种间变异与种内变异无重叠及条形码间隙;ITS1的种间变异与种内变异出现重叠。在亚种层面,12S、ITS、ITS1和ITS2的亚种间变异与亚种内变异出现重叠;16S的亚种间变异与亚种内变异无重叠及条形码间隙;t RNAs与COI序列亚种间变异与亚种内变异之间有明显的条形码间隙,有利于区分亚种。【结论】t RNAs和COI序列可用于种及亚种的鉴定。

关键词(KeyWords)： 巨砺属牡蛎;DNA条形码;线粒体DNA;核糖体RNA基因

Abstract:

Keywords:

基金项目(Foundation): 广西创新驱动发展专项资金项目(桂科AA17204088-1);; 农业基础性长期性科技工作国家渔业科学数据中心观测检测任务(ZX08S211664)

作者(Author): 彭敏,罗邦,朱威霖,杨春玲,赵永贞,李强勇,刘青云,曾地刚,李旻,陈秀荔

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