张雨婷,曾嫚玉,马佳仪,李嘉欣,焦钰,杜晓东

• 1:广东海洋大学水产学院
• 2:广东省珍珠养殖与加工工程技术研究中心

摘要(Abstract)：

【目的】研究马氏珠母贝(Pinctada martensii)β2肾上腺素能受体(Pmβ2AR)的生物学功能。【方法】利用cDNA末端快速扩增技术(RACE)克隆获得Pmβ2AR基因的cDNA的全长序列并对其序列特征进行分析,实时荧光定量技术(q RT-PCR)检测马氏珠母贝不同组织中β2AR基因m RNA的表达水平。【结果与结论】Pmβ2AR的cDNA全长2 426 bp,包括开放阅读框(ORF)2 091 bp,编码696个氨基酸,5′UTR长144 bp,3′UTR长191bp,预测分子质量79.0 ku,理论等电点9.18,多重序列比对结果发现β2AR在不同物种间的保守性较高。软件分析结果显示Pmβ2AR的氨基酸序列具有7个典型的跨膜结构域。q RT-PCR结果表明,Pmβ2AR基因在各组织中均有表达,在鳃中表达量最高,在肝胰腺和性腺中表达量也较高。

关键词(KeyWords)： 马氏珠母贝;β2肾上腺素能受体;分子特征;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31672626)

作者(Author): 张雨婷,曾嫚玉,马佳仪,李嘉欣,焦钰,杜晓东

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