符虹宇,李雁群,蔡奇珍,梁皓辉,黎钊坪,胡雪琼

• 1:广东海洋大学食品科技学院
• 2:广东海洋大学食品科技学院海洋药物研究所
• 3:广东省水产品加工与安全重点实验室

摘要(Abstract)：

【目的】探讨蛋白核小球藻采收后培养液是否能循环再用于小球藻的培养。【方法】采用小球藻分批培养完成采收细胞后所得培养废液来配制培养基,重新进入下一批次小球藻培养。以藻类BG11培养基配方为基准,按照配方全部或部分补充营养盐,通过测定藻液的光密度值间接反映藻生物量,比较小球藻在循环培养中的生长情况。【结果】在连续循环利用回收水至第4批次培养收获时培养液的光密度D(600 nm)为9.9,比生长速率为0.80 d-1,与第1批培养收获时培养液的光密度D(600 nm)和比生长速率相比,小球藻细胞产量和生长速率均未受影响。经过4个轮次的循环培养,用水量可减少约75%。在连续循环培养中仅补充N、P、S盐,至第3个循环小球藻收获时的生物量变化在10%以内。【结论】在加糖混养、保持连续光照、按照BG11配方补全营养盐的培养条件下,至少可对蛋白核小球藻进行4个周期的循环培养。按照BG11配方量补充相应的N、P、S盐,减少其他营养盐的投放,在2个循环周期内可以维持蛋白核小球藻的正常生长。利用培养废液实现小球藻循环经济培养和减少生产排放方法可行。

关键词(KeyWords)： 蛋白核小球藻;微藻培养;培养液循环

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金(2015A030313616);; 广东省国际合作项目(2017A050501038);; 湛江市科技计划项目(2015A03026)

作者(Author): 符虹宇,李雁群,蔡奇珍,梁皓辉,黎钊坪,胡雪琼

参考文献(References)：

• [1]韦金河,汪廷,冯伟民,等.小球藻大面积养殖技术[J].江苏农业科学,2004(2):74-76.
• [2]李师翁,李虎乾.植物单细胞蛋白资源--小球藻开发利用研究的现状[J].生物技术,1997(3):45-48.
• [3]王宝贝,蔡舒琳,李丽婷,等.小球藻在食品中的应用研究进展[J].食品工业科技,2017,38(17):347-352;358.
• [4]张旗,尚小玉,王娜,等.小球藻营养活性研究进展[J].食品研究与开发,2015,36(13):139-143.
• [5]PANAHI Y,DARVISHI B,JOWZI N,et al.Chlorella vulgaris:A multifunctional dietary supplement with diverse medicinal properties[J].Current Pharmaceutical Design,2016,22(2):164-173.
• [6]MORAIS M G D,COSTA J A V.Carbon dioxide fixation by Chlorella kessleri,C.vulgaris,Scenedesmus obliquus and Spirulina sp.cultivated in flasks and vertical tubular photobioreactors[J].Biotechnology Letters,2007,29(9):1349-1352.
• [7]XU H,MIAO X L,WU Q Y.High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters[J].Journal of Biotechnology,2006,126(4):499-507.
• [8]常杰.小球藻在动物营养中的应用研究进展[J].饲料研究,2016(2):7-10.
• [9]高攀峰,温小娟,沈惜坤,等.小球藻育藻条件优化及其在高位池对虾养殖中的应用[J].福建农业学报,2018,33(5):463-468.
• [10]朱顺妮,刘芬,樊均辉,等.微藻生物能源研究现状及展望[J].新能源进展,2018,6(6):467-474.
• [11]朱晓明,刘德富,杨正健,等.不同浓度培养液对小球藻生长的影响[J].环境科学与技术,2014,37(S2):51-57.
• [12]BARSANTI L,GUALTIERI P.Is exploitation of microalgae economically and energetically sustainable?[J].Algal Research,2018,31:107-115.
• [13]LARKUM A W D,ROSS I L,KRUSE O,et al.Selection,breeding and engineering of microalgae for bioenergy and biofuel production[J].Trends in Biotechnology,2012,30(4):198-205.
• [14]WANG Y,LING C,CHEN Y,et al.Microbial engineering for easy downstream processing[J].Biotechnology Advances,in press.DOI:10.1016/j.biotechadv.2019.03.004.
• [15]ANTONIA M,FILIPPOS V.Microalgal biofilms:Afurther step over current microalgal cultivation techniques[J].Science of The Total Environment,2019,651(2):3187-3201.
• [16]ZHUANG L L,YU D,ZHANG J,et al.The characteristics and influencing factors of the attached microalgae cultivation:A review[J].Renewable and Sustainable Energy Reviews,2018,94:1110-1119.
• [17]MENEGAZZO M L,FONSECA G G.Biomass recovery and lipid extraction processes for microalgae biofuels production:A review[J].Renewable and Sustainable Energy Reviews,2019,107:87-107.
• [18]WAN C,ALAM M A,ZHAO X Q,et al.Current progress and future prospect of microalgal biomass harvest using various flocculation technologies[J].Bioresource Technology,2015,184:251-257.
• [19]PRAGYA N,PANDEY K K,SAHOO P K.A review on harvesting,oil extraction and biofuels production technologies from microalgae[J].Renewable and Sustainable Energy Reviews,2013,24:159-171.
• [20]BENNAMOUN L,AFZAL M T,L ONARD A.Drying of alga as a source of bioenergy feedstock and food supplement-A review[J].Renewable and Sustainable Energy Reviews,2015,50:1203-1212.
• [21]张方,熊绍专,何加龙,等.用于生物柴油生产的微藻培养技术研究进展[J].化学与生物工程,2018,35(1):5-11.
• [22]BILAD M R,VANDAMME D,FOUBERT I,et al.Harvesting microalgal biomass using submerged microfiltration membranes[J].Bioresource Technology,2012,111:343-352.
• [23]GUIEYSSE B,B CHET Q,SHILTON A.Variability and uncertainty in water demand and water footprint assessments of fresh algae cultivation based on case studies from five climatic regions[J].Bioresource Technology,2013,128:317-323.
• [24]YANG J,XU M,ZHANG X Z,et al.Life-cycle analysis on biodiesel production from microalgae:Water footprint and nutrients balance[J].Bioresour Technol,2011,102(1):159-165.
• [25]MONTE J,SA M,PARREIRA C,et al.Recycling of Dunaliella salina cultivation medium by integrated membrane filtration and advanced oxidation[J].Algal Research,in press.DOI:10.1016/j.algal.2019.101460.
• [26]FRET J,ROEF L,BLUST R,et al.Reuse of rejuvenated media during laboratory and pilot scale cultivation of Nannochloropsis sp.[J].Algal Research,2017,27:265-273.
• [27]FAROOQ W,MOON M,RYU B G,et al.Effect of harvesting methods on the reusability of water for cultivation of Chlorella vulgaris,its lipid productivity and biodiesel quality[J].Algal Research,2015,8:1-7.
• [28]DEPRAETERE O,PIERRE G,NOPPE W,et al.Influence of culture medium recycling on the performance of Arthrospira platensis cultures[J].Algal Research,2015,10:48-54.
• [29]DENG X Y,GAO K,ADDY M,et al.Cultivation of Chlorella vulgaris on anaerobically digested swine manure with daily recycling of the post-harvest culture broth[J].Bioresource Technology,2018,247:716-723.