单甘酯与蜂蜡质量比、加热温度和时间对鱼油凝胶特性与抗氧化的影响Effect of Mass Ratio of Monoglyceride to Beeswax, Heating Temperature and Time on Characteristics and Antioxidant Capability of Fish Oil Oleogels
陈菲,沈丽平,韩宗元,刘阳,夏秋瑜,王泽富,夏文,刘书成
摘要(Abstract):
【目的】明确单甘酯与蜂蜡共组装制备鱼油凝胶的工艺条件,解决鱼油因富含ω-3多不饱和脂肪酸而易氧化酸败的问题。【方法】采用单因素实验考察单甘酯与蜂蜡质量比(10∶0、7∶3、5∶5、3∶7、0∶10)、加热温度(50、55、60、65℃)和时间(10、15、20 min)对鱼油凝胶特性及抗氧化能力的影响。【结果】单甘酯与蜂蜡共组装的鱼油凝胶具有较好的凝胶特性和抗氧化效能,特别是当单甘酯与蜂蜡质量比为3∶7时,在60℃加热10~20 min形成的鱼油凝胶,外观均匀光滑,持油率高达100%,硬度与凝胶强度较高,表现为弹性主导的假塑性流体,即储能模量(G′)大于损耗模量(G″),且G′最高,形成细长、密集、稳定的晶体网络结构,有效限制油脂流动。凝胶化过程会轻微促进鱼油初级氧化,但过氧化值和茴香胺值远低于二级精制鱼油标准限值,其中单甘酯与蜂蜡共组装的鱼油凝胶(尤其是3∶7组)的过氧化值(0.564 g/kg)和茴香胺值(0.228 7)均显著低于单甘酯或蜂蜡组装的鱼油凝胶(P<0.05),符合一级精制鱼油标准。【结论】通过单甘酯与蜂蜡共组装构建具有高氧化稳定性和应用性能的鱼油凝胶,为鱼油提供有效的物理抗氧化屏障。
关键词(KeyWords): 鱼油凝胶;单甘酯;蜂蜡;共组装;凝胶结构;抗氧化能力
基金项目(Foundation): 国家自然科学基金青年项目(32202086);; 广东省重点研发计划(2025B1111140001);; 广东海洋大学学生创新团队项目(CXTD2025002)
作者(Author): 陈菲,沈丽平,韩宗元,刘阳,夏秋瑜,王泽富,夏文,刘书成
参考文献(References):
- [1] FRYDRYCH A, KULITA K, JUROWSKI K, et al. Lipids in clinical nutrition and health:narrative review and dietary recommendations[J]. Foods, 2025, 14(3):473.
- [2] MIYASHITA K, UEMURA M, HOSOKAWA M. Effective prevention of oxidative deterioration of fish oil:focus on flavor deterioration[J]. Annual Review of Food Science and Technology, 2018, 9:209-226.
- [3] CHEN Y F, SUN Y, DING Y T, et al. Recent progress in fish oil-based emulsions by various food-grade stabilizers:Fabrication strategy, interfacial stability mechanism and potential application[J]. Critical Reviews in Food Science and Nutrition, 2022, 64(6):21-24.
- [4]张天恒,王立,李言.微胶囊技术在粮油食品加工中的应用进展[J].中国粮油学报, 2025, 40(3):209-218.
- [5] ZHOU Q, WEI Z H. Food-grade systems for delivery of DHA and EPA:Opportunities, fabrication, characterization and future perspectives[J]. Critical Reviews in Food Science and Nutrition, 2021, 63(15):11-18.
- [6] MANZOOR S, MASOODI F A, NAQASH F, et al. Oleogels:Promising alternatives to solid fats for food applications[J]. Food Hydrocolloids for Health, 2022, 2:100058.
- [7] GUO J X, CUI L J, MENG Z. Oleogels/emulsion gels as novel saturated fat replacers in meat products:a review[J].Food Hydrocolloids, 2023, 137:108313.
- [8] LEE M C, TAN C, ABBASPOURRAD A. Combination of internal structuring and external coating in an oleogelbased delivery system for fish oil stabilization[J]. Food Chemistry, 2019, 277:213-221.
- [9] MALVANO F, ALBANESE D, CINQUANTA L, et al. A comparative study between beeswax and glycerol monostearate for food-grade oleogels[J]. Gels, 2024, 10(4):214.
- [10] SHUAI X X, MCCLEMENTS D J, GENG Q, et al. Macadamia oil-based oleogels as cocoa butter alternatives:Physical properties, oxidative stability, lipolysis, and application[J]. Food Research International, 2023, 172:113098.
- [11] HWANG H S, FHANER M, WINKLER-MOSER J K, et al. Oxidation of fish oil oleogels formed by natural waxes in comparison with bulk oil[J]. European Journal of Lipid Science and Technology, 2018, 120(5):1700378.
- [12] PALLA C A, DOMINGUEZ M, CARRíN M E. Recent advances on food-based applications of monoglyceride oleogels[J]. Journal of the American Oil Chemists'Society,2022, 99(11):985-1006.
- [13] SUN H, XU J, LU X Z, et al. Development and characterization of monoglyceride oleogels prepared with crude and refined walnut oil[J]. LWT, 2022, 154:112769.
- [14] MA Y F, JIANG Q B, ZHENG S J, et al. Physical properties and stability of oleogels and oleogel emulsions based on human milk mimic oil[J]. ACS Food Science&Technology, 2024, 4(4):992-1000.
- [15]张瑜,薛雨舒,沈乙杰,等.超声处理对蜂蜡-单甘酯基核桃油凝胶结构与性质的影响[J].轻工学报, 2024, 39(1):12-21.
- [16] JEONG S, OH I. Characterization of mixed-component oleogels:Beeswax and glycerol monostearate interactions towards Tenebrio molitor larvae oil[J]. Current Research in Food Science, 2024, 8:100689.
- [17] DIMAKOPOULOU-PAPAZOGLOU D, ZAMPOUNI K,PRODROMIDIS P, et al. Microstructure, physical properties, and oxidative stability of olive oil oleogels composed of sunflower wax and monoglycerides[J]. Gels, 2024, 10(3):195.
- [18] CHENG K X, PAN Y M, HAN Z Y, et al. A sight of selfassembly mechanism in fish oil oleogels:Phase transition,crystal structure and non-covalent interaction[J]. Food Chemistry, 2024, 433:137323.
- [19]刘书成,马健康,王蔚雯,等.蜂蜡添加量对鱼油凝胶宏观性质及微观结构的影响[J].中国食品学报, 2024, 24(4):90-98.
- [20]张灵枝.分光光度法测定油脂氧化物的过氧化值[J].现代食品, 2019, 25(11):169-171.
- [21]韩宗元,刘雨杉,梁嘉翰,等.冷却方式对蜂蜡基鱼油凝胶宏观性质与微观结构的影响[J].广东海洋大学学报,2023, 43(5):60-66.
- [22] YLMAZ E,??üTCüM. Properties and stability of hazelnut oil organogels with beeswax and monoglyceride[J]. Journal of the American Oil Chemists'Society, 2014,91(6):1007-1017.
- [23]张园园,李建芳.信阳地区茶油基油凝胶的制备及特性分析[J].中国食品添加剂, 2023, 34(2):191-196.
- [24] FADILA M S, AL AWWALY K U, MANAB A, et al.Development and characterization of bigel based on corn oil and beeswax oleogel with gelatin hydrogel as a fat replacer[J]. Asian Food Science Journal, 2025, 24(5):51-61.
- [25] SHUAI X X, JULIAN MCCLEMENTS D, DAI T T, et al.Effect of different oleogelators on physicochemical properties, oxidative stability and astaxanthin delivery of Macadamia oil-based oleogels[J]. Food Research International, 2024, 196:115131.
- [26] YI H J, KANG Y R, CHANG Y H. Structural and rheological properties of bigels formed with xanthan gum hydrogel and lecithin/glycerol monostearate oleogel[J].International Journal of Biological Macromolecules, 2025,306:141549.
- [27] LI J X, GUO R H, WANG M X, et al. Development and characterization of compound oleogels based on monoglycerides and edible waxes[J]. ACS Food Science&Technology, 2022, 2(2):302-314.
- [28] ZHANG Y Z, XU J Q, TANG C E, et al. Crystallization behavior and physical properties of monoglycerides-based oleogels as function of oleogelator concentration[J].Foods, 2023, 12(2):345.
- [29]李秀芬,赵冰,罗嘉,等.可食用油凝胶的流变-结晶特性研究进展[J].食品研究与开发, 2023, 44(7):175-184.
- [30] PANG M, XU L, WANG Y G, et al. Potential functional oleogels based on phytosterol and diacylglycerol corn oil:Development and physicochemical characterization[J].LWT, 2024, 198:115987.
- [31] DIMAKOPOULOU-PAPAZOGLOU D, GIANNAKAKI F, KATSANIDIS E. Structural and physical characteristics of mixed-component oleogels:natural wax and monoglyceride interactions in different edible oils[J]. Gels, 2023, 9(8):627.
- [32] FROLOVA Y V, SOBOLEV R V, SARKISYAN V A, et al.Approaches to study the oxidative stability of oleogels[J].IOP Conference Series:Earth and Environmental Science,2021, 677(3):032045.
- [33] COELHO A L K, DE ANDRADE BARBOSA GUILHERME M, DE FREITAS R A, et al. Tunable oleogels from sunflower oil, ethylcellulose, and Quillaja saponin:Synergistic interactions, structural properties, and oxidative stability[J]. Food Hydrocolloids, 2026, 170:111744.
- [34] SUN X L, WANG Y J, LI H K, et al. Changes in the volatile profile, fatty acid composition and oxidative stability of flaxseed oil during heating at different temperatures[J].LWT, 2021, 151:112137.