夏洪丽,鲁义善,汤菊芬,蔡佳,汪美,夏立群

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点实验室
• 3:广东省教育厅水产经济动物病害控制重点实验室

摘要(Abstract)：

鱼类诺卡氏菌病是全球性的鱼病,杀鲑诺卡氏菌(Nocardia salmonicida)是引起鱼类诺卡氏菌病的主要病原之一。根据杀鲑诺卡氏菌16S-23S转录间隔区(ITS)序列设计引物,建立特异性PCR检测杀鲑诺卡氏菌的方法。结果表明,筛选的PCR引物可特异性地扩增出杀鲑诺卡氏菌的ITS片段,检测灵敏度(DNA浓度)为28.3 pg·μL-1。检测人工感染杀鲑诺卡氏菌的鱼组织,结果显示,该方法可从未分离到病原菌的病鱼组织中检出阳性片段,较传统细菌分离鉴定方法更为高效灵敏。

关键词(KeyWords)： 杀鲑诺卡氏菌;转录间隔区;聚合酶链式反应;检测

Abstract:

Keywords:

基金项目(Foundation): 农业部公益性行业科研专项(201203085);; 湛江市科技计划项目(2013B01001);; 广东海洋大学引起人才科研启动费(C13454);; 大学生创新训练项目(1056613015)

作者(Author): 夏洪丽,鲁义善,汤菊芬,蔡佳,汪美,夏立群

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