张智超,陈永坤,罗子皓,罗植森,林弘都,何雄波,颜云榕

• 1:广东海洋大学水产学院

摘要(Abstract)：

【目的】分析我国7个点带石斑鱼（Epinephelus coioides）养殖群体的遗传多样性及遗传结构，为全面了解我国点带石斑鱼养殖群体的遗传现状，选育石斑鱼优良品种提供科学依据。【方法】采集海南陵水、儋州，广东惠阳、阳西（阳江）、江城（阳江），福建东山等6地区点带石斑鱼7群体的鳍条或肌肉样品，采用全基因组重测序技术，筛选出高质量单核苷酸多态性（SNPs），对7群体105尾个体进行遗传多样性及群体结构评估。【结果与结论】测序共产生1 206.82 Gb碱基数据，经质控筛选过滤后获得8 327 608个高质量SNPs。各群体期望杂合度（He）为0.349 7～0.351 9，核苷酸多样性（π）为0.002 3～0.002 5，多态信息含量（PIC）为0.263 9～0.275 9，表明各群体遗传多样性均处于中等水平，各养殖群体的遗传多样性水平整体差异较小；所有群体的连锁不平衡衰减较快，表明群体的驯化程度低；遗传分化指数（Fst）平均值仅为0.011 4，说明遗传分化程度极低。遗传结构分析表明，所有群体划分为2个祖先群，各群体在进化树和主成分分析上无明显的群体聚类，表明群体间结构组成相似；群体间有2次基因流动，说明存在共同遗传混合事件。研究结果可为我国点带石斑鱼种质资源保护、亲本选育及科学养殖等提供重要的理论依据。

关键词(KeyWords)： 点带石斑鱼;全基因组重测序;单核苷酸多态性;遗传多样性;群体结构

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发计划（2022YFD2401300）;; 海洋牧场野外科学观测研究站项目（2024B1212040002）;; 青年科学基金（42406098）

作者(Author): 张智超,陈永坤,罗子皓,罗植森,林弘都,何雄波,颜云榕

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