谢丽施,张宁,李长玲,黄翔鹄

• 1:广东海洋大学深圳研究院
• 2:广东海洋大学水产学院
• 3:广东省藻类养殖及应用工程技术研究中心

摘要(Abstract)：

【目的】研究硝酸钠对波吉卵囊藻(Oocystis borgei)生长、生化组分及沉降的影响。【方法】采用分光光度计法、乙醇法、尼罗红染色法、苯酚硫酸法和考马斯亮蓝法,分别测定波吉卵囊藻在不同硝酸钠浓度下的生物量、叶绿素、脂质、总糖和蛋白质含量,分析波吉卵囊藻沉降与生长及生化组分的关系。【结果】1)硝酸钠对波吉卵囊藻沉降率有显著影响(P <0.05),当硝酸钠74.8 mg/L时,沉降6 h的沉降率达到75%,沉降效果最佳。2)硝酸钠对波吉卵囊藻生物量和叶绿素的影响显著(P <0.05),培养9 d时,硝酸钠37.4～748.0 mg/L组卵囊藻生物量和叶绿素含量较高,生长状态好,沉降率较高;经回归分析,沉降率与生物量呈正相关关系(R~2=0.824 3),与叶绿素含量呈先增加后减少的抛物线关系(R~2=0.819 8)。3)硝酸钠对波吉卵囊藻脂质和总糖含量的影响显著(P<0.05),硝酸钠质量浓度为37.4～2 992.0 mg/L时,总糖和脂质含量少,沉降率较高;经一元回归分析,沉降率与脂质和总糖含量呈负相关关系,R~2分别为0.770 5和0.609 4。4)沉降率与生物量和叶绿素有显著正相关性,皮尔逊系数r分别为0.785和0.404;与脂质和总糖有显著负相关性,皮尔逊系数r分别为―0.788和―0.731。【结论】硝酸钠通过影响波吉卵囊藻的生长和生化组分来改变藻细胞的沉降率;波吉卵囊藻的沉降率与生长状态呈正相关关系,与脂质、总糖呈负相关关系,脂质和总糖含量越少,沉降效果越好,与蛋白质无显著相关关系。

关键词(KeyWords)： 波吉卵囊藻;硝酸钠;生化组分;沉降

Abstract:

Keywords:

基金项目(Foundation): 深圳市大鹏新区产业发展专项资金“活性微藻制品产业化关键技术的研究与示范”(KY20180112);; 广东海洋大学博士启动项目

作者(Author): 谢丽施,张宁,李长玲,黄翔鹄

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