高风英,王欢,叶星,卢迈新,黄樟翰,朱华平

• 1:中国水产科学研究院珠江水产研究所
• 2:广东海洋大学水产学院

摘要(Abstract)：

用RT-PCR和RACE方法,从荷那龙罗非鱼(Oreochromis hornorum)垂体中克隆到生长激素促分泌素受体(GHSR)cDNA全序列。荷那龙罗非鱼GHSR基因具有GHSR-1a与GHSR-1b两个高度保守cDNA序列。GHSR-1a序列全长1 646 bp,包括225 bp的5′非编码区,266 bp的3′非编码区和1 155 bp的开放阅读框,编码384个氨基酸残基,具有7个跨膜结构域结构(transmembrane domains,TM);GHSR-1b序列全长1 877 bp,包括225 bp的5′非编码区,755 bp的3′非编码区和897 bp的开放阅读框,编码298个氨基酸残基,只具有前5个TM,在第6个TM的第4个氨基酸处开始缺失。将GHSR cDNA序列与基因组序列比较发现,这两种cDNA转录本来自同一个基因的不同变体。

关键词(KeyWords)： 荷那龙罗非鱼;生长激素促分泌素受体(GHSR);基因克隆;基因序列

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项资金(CARS-49);; 广东省农业重点项目(2009B020201003);; 广东省海洋渔业科技推广专项(A200901C01)

作者(Author): 高风英,王欢,叶星,卢迈新,黄樟翰,朱华平

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